New PDF release: Advances in lipid research. Volume 6

By Rodolfo Paoletti, Dr. David Kritchevsky

ISBN-10: 1483199428

ISBN-13: 9781483199429

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Therefore, the use of fasting samples enhances both the accuracy and the reproducibility of the data and per- 30 F. T. HATCH AND R. S. LEES mits valid comparisons with published information obtained under similar conditions. C. P R E P A R A T I O N O F S E R U M O R P L A S M A F O R ANALYSIS Lipoproteins are moderately unstable substances and become particularly so when, during fractionation procedures, they become separated from their normal environment of serum proteins and small molecules.

C. P R E P A R A T I O N O F S E R U M O R P L A S M A F O R ANALYSIS Lipoproteins are moderately unstable substances and become particularly so when, during fractionation procedures, they become separated from their normal environment of serum proteins and small molecules. , 1954). Although the molecular mechanisms of deterioration are not understood, the result is a progressive alteration in chemical, ultracentrifugal, or electrophoretic behavior. A plasma enzyme which transfers fatty acids from phospholipid to cholesterol (Glomset, 1962) and a system which exchanges lipids between H D L and VLDL (Nichols and Smith, 1965) may contribute other changes in lipoprotein composition and properties during storage.

H A T C H AND R. S. L E E S The principal lipoprotein zones are illustrated in Fig. 4 and compared with the serum protein pattern as it is observed in paper electrophoresis. The correspondence between the electrophoretic lipoprotein zones and the standard ultracentrifugal fractions is given in Table V. , 1968). 019) exhibit an electrophoretic mobility between that of ß- and pre-ß-lipoproteins. However, no practicable estimation of this class by means of paper electrophoresis is possible, and no special clinical significance for it is known.

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Advances in lipid research. Volume 6 by Rodolfo Paoletti, Dr. David Kritchevsky


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