By Tanya E. S. Dahms, Kirk J. Czymmek
The goal of this quantity is to explain the most recent advances in microscopic tools, together with built-in recommendations, as utilized to mycology. each one bankruptcy will offer a short evaluation of a selected microscopic procedure with linked merits and boundaries, the examine questions that may be accurately addressed utilizing those microscopic equipment, the way it has been effectively utilized to deal with mycological learn questions, together with aiding and complimentary ideas, and which destiny questions will be addressed.
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Extra info for Advanced Microscopy in Mycology
Bright organic dyes can be delivered via antibodies. Nanobodies are a unique form of monoclonal antibodies, characterized by a single antigen-binding domain (12– 15 kDa) present in camels (Hamers-Casterman et al. 1993). The nanobody can be easily produced in bacteria or yeast and binds to the antigen with high affinity and specificity. The diverse application of nanobodies will be important in general cell biology (Ries et al. 2012). Overall, the advanced microscopy techniques reviewed in this chapter open new avenues for experimentation that promise to generate key information on the molecular mechanisms that control signal transduction, morphogenesis, and development of filamentous fungi.
J Cell Sci 115:1931–1937 Haase J, Mishra PF, Stephens A, Haggerty R, Quammen C, Taylor RM, Yeh E, Basrai MA, Bloom K (2013) A 3D map of the yeast kinetochore reveals the presence of core and accessory centromere-specific histone. Curr Biol 23:1939–1944 Haraguchi T, Shimi T, Koujin T, Hashiguchi N, Hiraoka Y (2002) Spectral imaging fluorescence microscopy. Genes Cells 7:881–887 Hibbs AR (2004) Confocal microscopy for biologists. Springer, New York Hickey PC, Read ND (2003) Biology of living fungi.
Nat Methods 2:920–931 Czymmek KJ (2005) Exploring fungal activity with confocal and multiphoton microscopy. In Dighton J, White JF, Oudemans P (eds) The fungal community: its organization and role in the ecosystem, 3rd edn. CRC Press, Boca Raton Czymmek KJ, Whallon JH, Klomparens A (1994) Confocal microscopy in mycological research. ExpMycol 18:275–293 22 R. R. Mouriño-Pérez and R. W. Roberson Czymmek KJ, Fogg M, Powell DH, Sweigard J, Park SY, Kang S (2007) In vivo time-lapse documentation using confocal and multi-photon microscopy reveals the mechanisms of invasion into the Arabidopsis root vascular system by Fusarium oxysporum.
Advanced Microscopy in Mycology by Tanya E. S. Dahms, Kirk J. Czymmek